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A review of manufacturing capabilities of cell spheroid generation technologies and future development
Author(s) -
Liu Dan,
Chen Sixun,
Win Naing May
Publication year - 2021
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.27620
Subject(s) - spheroid , standardization , scalability , nanotechnology , computer science , process (computing) , cell , biochemical engineering , cell culture , materials science , biological system , chemistry , biology , engineering , genetics , biochemistry , database , operating system
Spheroid culture provides cells with a three‐dimensional environment that can better mimic physiological conditions compared to monolayer culture. Technologies involved in the generation of cell spheroids are continuously being innovated to produce spheroids with enhanced properties. In this paper, we review the manufacturing capabilities of current cell spheroid generation technologies. We propose that spheroid generation technologies should enable tight and robust process controls to produce spheroids of consistent and repeatable quality. Future technology development for the generation of cell spheroids should look into improvement in process control, standardization, scalability and monitoring, in addition to advanced methods of spheroid transfer and characterization.