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Ferric ion crosslinking‐based 3D printing of a graphene oxide hydrogel and its evaluation as a bio‐scaffold in tissue engineering
Author(s) -
Lu Renhao,
Zhang Wuhua,
He Yuting,
Zhang Siyuan,
Fu Qian,
Pang Yuan,
Sun Wei
Publication year - 2021
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.27592
Subject(s) - materials science , graphene , tissue engineering , scaffold , nanotechnology , ferric , biocompatibility , oxide , fabrication , 3d printing , chemical engineering , extrusion , biomedical engineering , composite material , metallurgy , medicine , alternative medicine , engineering , pathology
As a precursor of graphene, graphene oxide (GO) exhibits excellent mechanical, thermal, and electrical properties, besides appreciable biocompatibility in tissue engineering applications. However, the current GO‐3D fabrication technology is still in need of optimization and simplification to ensure fine architecture and reasonable mechanical properties, which would further promote the performance of GO as bio‐scaffolds in cell or microorganism attachment and in material transformation. To address this issue, we proposed a GO ink, with appreciable rheological properties and excellent printing performance via high‐speed centrifugation and ferric ion‐assisted cross‐linking. A woodpile structure with controllable micro‐pores was produced by micro‐extrusion‐based 3D printing technology followed by an optimized freeze‐drying process. Cellular adhesion and viability were verified by inoculation and culture of HepaRG cells using the fabricated GO 3D structure, thus suggesting ferric ion‐assisted cross‐linking and controllable pore distribution for improving the performance of the GO construct as a bio‐scaffold for in vitro liver tissue models.