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Consolidated bioprocessing performance of a two‐species microbial consortium for butanol production from lignocellulosic biomass
Author(s) -
Jiang Yujia,
Lv Yang,
Wu Ruofan,
Lu Jiasheng,
Dong Weiliang,
Zhou Jie,
Zhang Wenming,
Xin Fengxue,
Jiang Min
Publication year - 2020
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.27464
Subject(s) - bioprocess , xylan , butanol , xylose , hemicellulose , clostridium acetobutylicum , chemistry , lignocellulosic biomass , xylanase , biomass (ecology) , food science , biochemistry , microbial consortium , fermentation , hydrolysis , microbiology and biotechnology , enzyme , biology , microorganism , bacteria , ethanol , agronomy , paleontology , genetics
Abstract Consolidated bioprocessing (CBP) by using microbial consortium was considered as a promising approach to achieve direct biofuel production from lignocellulose. In this study, the interaction mechanism of microbial consortium consisting of Thermoanaerobacterium thermosaccharolyticum M5 and Clostridium acetobutylicum NJ4 was analyzed, which could achieve efficient butanol production from xylan through CBP. Strain M5 possesses efficient xylan degradation capability, as 19.73 g/L of xylose was accumulated within 50 hr. The efficient xylose utilization capability of partner strain NJ4 could relieve the substrate inhibition to hydrolytic enzymes of xylanase and xylosidase secreted by strain M5. In addition, the earlier solventogenesis of strain NJ4 was observed due to the existence of butyrate generated by strain M5. The mutual interaction of these two strains finally gave 13.28 g/L of butanol from 70 g/L of xylan after process optimization, representing a relatively high butanol production from hemicellulose. Moreover, 7.61 g/L of butanol was generated from untreated corncob via CBP. This successfully constructed microbial consortium exhibits efficient cooperation performance on butanol production from lignocellulose, which could provide a platform for the emerging butanol production from lignocellulose.