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Real‐time dissolved carbon dioxide monitoring II: Surface aeration intensification for efficient CO 2 removal in shake flasks and mini‐bioreactors leads to superior growth and recombinant protein yields
Author(s) -
Chopda Viki R.,
Holzberg Timothy,
Ge Xudong,
Folio Brandon,
Wong Lynn,
Tolosa Michael,
Kostov Yordan,
Tolosa Leah,
Rao Govind
Publication year - 2020
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.27252
Subject(s) - aeration , laboratory flask , bioreactor , sparging , shake , carbon dioxide , biomass (ecology) , pulp and paper industry , bioprocess , chemistry , environmental science , chromatography , chemical engineering , biology , ecology , organic chemistry , physics , astronomy , engineering
Mass transfer is known to play a critical role in bioprocess performance and henceforth monitoring dissolved O 2 (DO) and dissolved CO 2 (dCO 2 ) is of paramount importance. At bioreactor level these parameters can be monitored online and can be controlled by sparging air/oxygen or stirrer speed. However, traditional small‐scale systems such as shake flasks lack real time monitoring and also employ only surface aeration with additional diffusion limitations imposed by the culture plug. Here we present implementation of intensifying surface aeration by sparging air in the headspace of the reaction vessel and real‐time monitoring of DO and dCO 2 in the bioprocesses to evaluate the impact of intensified surface aeration. We observed that sparging air in the headspace allowed us to keep dCO 2 at low level, which significantly improved not only biomass growth but also protein yield. We expect that implementing such controlled smart shake flasks can minimize the process development gap which currently exists in shake flask level and bioreactor level results.