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Intestine‐on‐chip device increases ECM remodeling inducing faster epithelial cell differentiation
Author(s) -
De Gregorio Vincenza,
Corrado Brunella,
Sbrescia Simone,
Sibilio Sara,
Urciuolo Francesco,
Netti Paolo A.,
Imparato Giorgia
Publication year - 2020
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.27186
Subject(s) - extracellular matrix , microbiology and biotechnology , stromal cell , organ on a chip , basement membrane , cellular differentiation , chemistry , epithelium , mucus , microfluidics , biology , pathology , nanotechnology , cancer research , biochemistry , materials science , medicine , gene , ecology
An intestine‐on‐chip has been developed to study intestinal physiology and pathophysiology as well as intestinal transport absorption and toxicity studies in a controlled and human similar environment. Here, we report that dynamic culture of an intestine‐on‐chip enhances extracellular matrix (ECM) remodeling of the stroma, basement membrane production and speeds up epithelial differentiation. We developed a three‐dimensional human intestinal stromal equivalent composed of human intestinal subepithelial myofibroblasts embedded in their own ECM. Then, we cultured human colon carcinoma‐derived cells in both static and dynamic conditions in the opportunely designed microfluidic system until the formation of a well‐oriented epithelium. This low cost and handy microfluidic device allows to qualitatively and quantitatively detect epithelial polarization and mucus production as well as monitor barrier function and ECM remodeling after nutraceutical treatment.