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Techniques for chromosomal integration and expression optimization in Escherichia coli
Author(s) -
Ou Bingming,
Garcia Carolina,
Wang Yejun,
Zhang Weiping,
Zhu Guoqiang
Publication year - 2018
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.26790
Subject(s) - heterologous , escherichia coli , biology , heterologous expression , chromosome , computational biology , circular bacterial chromosome , metabolic engineering , gene , gene expression , synthetic biology , protein expression , genetics , microbiology and biotechnology , recombinant dna
Due to the inherent expression stability and low metabolic burden to the host cell, the expression of heterologous proteins in the bacterial chromosome in a precise and efficient manner is highly desirable for metabolic engineering and live bacterial applications. However, obtaining suitable chromosome expression levels is particularly challenging. In this minireview, we briefly present the technologies available for the integration of heterologous genes into Escherichia coli chromosomes and strategies to optimize the expression levels of heterologous proteins.