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Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid–derived hydrocarbons
Author(s) -
Zhang Yiming,
Nielsen Jens,
Liu Zihe
Publication year - 2018
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.26738
Subject(s) - yeast , saccharomyces cerevisiae , fatty acid , metabolic engineering , biochemistry , heterologous , biofuel , chemistry , metabolic pathway , biochemical engineering , microbiology and biotechnology , biology , metabolism , enzyme , engineering , gene
Abstract Fatty acid–derived hydrocarbons attract increasing attention as biofuels due to their immiscibility with water, high‐energy content, low freezing point, and high compatibility with existing refineries and end‐user infrastructures. Yeast Saccharomyces cerevisiae has advantages for production of fatty acid–derived hydrocarbons as its native routes toward fatty acid synthesis involve only a few reactions that allow more efficient conversion of carbon substrates. Here we describe major biosynthetic pathways of fatty acid–derived hydrocarbons in yeast, and summarize key metabolic engineering strategies, including enhancing precursor supply, eliminating competing pathways, and expressing heterologous pathways. With recent advances in yeast production of fatty acid–derived hydrocarbons, our review identifies key research challenges and opportunities for future optimization, and concludes with perspectives and outlooks for further research directions.