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Enhanced isobutanol production from acetate by combinatorial overexpression of acetyl‐CoA synthetase and anaplerotic enzymes in engineered Escherichia coli
Author(s) -
Song HunSuk,
Seo HyungMin,
Jeon JongMin,
Moon YuMi,
Hong Ju Won,
Hong Yoon Gi,
Bhatia Shashi Kant,
Ahn Jungoh,
Lee Hongweon,
Kim Wooseong,
Park YongCheol,
Choi KwonYoung,
Kim YunGon,
Yang YungHun
Publication year - 2018
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.26710
Subject(s) - isobutanol , metabolic engineering , biochemistry , chemistry , escherichia coli , enzyme , acetic acid , flux (metallurgy) , citric acid cycle , organic chemistry , ethanol , gene
Acetic acid is an abundant material that can be used as a carbon source by microorganisms. Despite its abundance, its toxicity and low energy content make it hard to utilize as a sole carbon source for biochemical production. To increase acetate utilization and isobutanol production with engineered Escherichia coli , the feasibility of utilizing acetate and metabolic engineering was investigated. The expression of acs, pckA , and maeB increased isobutanol production by up to 26%, and the addition of TCA cycle intermediates indicated that the intermediates can enhance isobutanol production. For isobutanol production from acetate, acetate uptake rates and the NADPH pool were not limiting factors compared to glucose as a carbon source. This work represents the first approach to produce isobutanol from acetate with pyruvate flux optimization to extend the applicability of acetate. This technique suggests a strategy for biochemical production utilizing acetate as the sole carbon source.