A microbial–enzymatic strategy for producing chondroitin sulfate glycosaminoglycans | Zendy

Zhou Zhengxiong | Zendy; Li Qing | Zendy; Huang Hao | Zendy; Wang Hao | Zendy; Wang Yang | Zendy; Du Guocheng | Zendy; Chen Jian | Zendy; Kang Zhen | Zendy

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A microbial–enzymatic strategy for producing chondroitin sulfate glycosaminoglycans

Author(s) -

Zhou Zhengxiong,

Li Qing,

Huang Hao,

Wang Hao,

Wang Yang,

Du Guocheng,

Chen Jian,

Kang Zhen

Publication year - 2018

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.26577

Subject(s) - chondroitin , chondroitin sulfate , sulfotransferase , sulfation , biochemistry , chemistry , glycosaminoglycan

Chondroitin sulfate has been widely used in both medical and clinical applications. Commercial chondroitin sulfate has been mainly acquired from animal tissue extraction. Here we report a new two‐step biological strategy for producing chondroitin sulfate A and chondroitin sulfate C. First, the chondroitin biosynthesis pathway in a recombinant Bacillus subtilis strain using sucrose as carbon source was systematically optimized and the titer of chondroitin was significantly enhanced to 7.15 g/L. Then, specific sulfation transformation systems were successfully constructed and optimized by combining the purified aryl sulfotransferase IV (ASST IV), chondroitin 4‐sulfotransferase (C4ST) and chondroitin 6‐sulfotransferase (C6ST). Chondroitin sulfate A and C were enzymatically transformed from chondroitin at conversion rates of 98% and 96%, respectively. The present biological strategy has great potential to be scaled up for biosynthesis of chondroitin sulfate A and C from cheap carbon sources.

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