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A microbial–enzymatic strategy for producing chondroitin sulfate glycosaminoglycans
Author(s) -
Zhou Zhengxiong,
Li Qing,
Huang Hao,
Wang Hao,
Wang Yang,
Du Guocheng,
Chen Jian,
Kang Zhen
Publication year - 2018
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.26577
Subject(s) - chondroitin , chondroitin sulfate , sulfotransferase , sulfation , biochemistry , chemistry , glycosaminoglycan
Chondroitin sulfate has been widely used in both medical and clinical applications. Commercial chondroitin sulfate has been mainly acquired from animal tissue extraction. Here we report a new two‐step biological strategy for producing chondroitin sulfate A and chondroitin sulfate C. First, the chondroitin biosynthesis pathway in a recombinant Bacillus subtilis strain using sucrose as carbon source was systematically optimized and the titer of chondroitin was significantly enhanced to 7.15 g/L. Then, specific sulfation transformation systems were successfully constructed and optimized by combining the purified aryl sulfotransferase IV (ASST IV), chondroitin 4‐sulfotransferase (C4ST) and chondroitin 6‐sulfotransferase (C6ST). Chondroitin sulfate A and C were enzymatically transformed from chondroitin at conversion rates of 98% and 96%, respectively. The present biological strategy has great potential to be scaled up for biosynthesis of chondroitin sulfate A and C from cheap carbon sources.