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3D aggregate culture improves metabolic maturation of human pluripotent stem cell derived cardiomyocytes
Author(s) -
Correia Cláudia,
Koshkin Alexey,
Duarte Patrícia,
Hu Dongjian,
Carido Madalena,
Sebastião Maria J.,
GomesAlves Patrícia,
Elliott David A.,
Domian Ibrahim J.,
Teixeira Ana P.,
Alves Paula M.,
Serra Margarida
Publication year - 2018
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.26504
Subject(s) - induced pluripotent stem cell , transcriptome , glycolysis , metabolic flux analysis , cell culture , microbiology and biotechnology , biology , flux (metallurgy) , oxidative phosphorylation , metabolic pathway , biochemistry , gene , chemistry , gene expression , metabolism , embryonic stem cell , genetics , organic chemistry
Three‐dimensional (3D) cultures of human pluripotent stem cell derived cardiomyocytes (hPSC‐CMs) hold great promise for drug discovery, providing a better approximation to the in vivo physiology over standard two‐dimensional (2D) monolayer cultures. However, the transition of CM differentiation protocols from 2D to 3D cultures is not straightforward. In this work, we relied on the aggregation of hPSC‐derived cardiac progenitors and their culture under agitated conditions to generate highly pure cardiomyocyte aggregates. Whole‐transcriptome analysis and 13 C‐metabolic flux analysis allowed to demonstrate at both molecular and fluxome levels that such 3D culture environment enhances metabolic maturation of hiPSC‐CMs. When compared to 2D, 3D cultures of hiPSC‐CMs displayed down‐regulation of genes involved in glycolysis and lipid biosynthesis and increased expression of genes involved in OXPHOS. Accordingly, 3D cultures of hiPSC‐CMs had lower fluxes through glycolysis and fatty acid synthesis and increased TCA‐cycle activity. Importantly, we demonstrated that the 3D culture environment reproducibly improved both CM purity and metabolic maturation across different hPSC lines, thereby providing a robust strategy to derive enriched hPSC‐CMs with metabolic features closer to that of adult CMs.