Premium
Types of cell death and apoptotic stages in Chinese Hamster Ovary cells distinguished by Raman spectroscopy
Author(s) -
Rangan Shreyas,
Kamal Sepehr,
Konorov Stanislav O.,
Schulze Hans Georg,
Blades Michael W.,
Turner Robin F. B.,
Piret James M.
Publication year - 2018
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.26476
Subject(s) - chinese hamster ovary cell , apoptosis , programmed cell death , biology , nucleic acid , microbiology and biotechnology , raman spectroscopy , cell , necrosis , cell culture , chemistry , biochemistry , genetics , physics , optics
Cell death is the ultimate cause of productivity loss in bioreactors that are used to produce therapeutic proteins. We investigated the ability of Raman spectroscopy to detect the onset and types of cell death for Chinese Hamster Ovary (CHO) cells—the most widely used cell type for therapeutic protein production. Raman spectroscopy was used to compare apoptotic, necrotic, autophagic, and control CHO cells. Several specific nucleic acid‐, protein‐, and lipid‐associated marker bands within the 650–850 cm −1 spectral region were identified that distinguished among cells undergoing different modes of cell death; supporting evidence was provided by principal component analysis (PCA) of the full spectral data. In addition to comparing the different modes of cell death, normal cells were compared to cells sorted at several stages of apoptosis, in order to explore the potential for early detection of apoptosis. Different stages of apoptosis could be distinguished via Raman spectroscopy, with multiple changes observed in nucleic acid peaks at early stages whereas an increase in lipid signals was a feature of late apoptosis/secondary necrosis.