Premium
Alginate‐gelatin encapsulation of human endothelial cells promoted angiogenesis in in vivo and in vitro milieu
Author(s) -
Nemati Sorour,
Rezabakhsh Aysa,
Khoshfetrat Ali Baradar,
Nourazarian Alireza,
Biray Avci Çığır,
Goker Bagca Bakiye,
Alizadeh Sardroud Hamed,
Khaksar Majid,
Ahmadi Mahdi,
Delkhosh Aref,
Sokullu Emel,
Rahbarghazi Reza
Publication year - 2017
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.26395
Subject(s) - in vivo , gelatin , angiogenesis , chemistry , in vitro , umbilical vein , transplantation , viability assay , microbiology and biotechnology , cell , biology , cancer research , biochemistry , medicine , surgery
Up to present, many advantages have been achieved in the field of cell‐based therapies by applying sophisticated methodologies and delivery approaches. Microcapsules are capable to provide safe microenvironment for cells during transplantation in a simulated physiological 3D milieu. Here, we aimed to investigate the effect of alginate‐gelatin encapsulation on angiogenic behavior of human endothelial cells over a period of 5 days. Human umbilical vein endothelial cells were encapsulated by alginate‐gelatin substrate and incubated for 5 days. MTT and autophagy PCR array analysis were used to monitor cell survival rate. For in vitro angiogenesis analysis, cell distribution of Tie‐1, Tie‐2, VEGFR‐1, and VEGFR‐2 were detected by ELISA. In addition to in vitro tubulogenesis assay, we monitored the expression of VE‐cadherin by Western blotting. The migration capacity of encapsulated HUVECs was studied by measuring MMP‐2 and MMP‐9 via gelatin zymography. The in vivo angiogenic potential of encapsulated HUVECs was analyzed in immune‐compromised mouse implant model during 7 days post‐transplantation. We demonstrated that encapsulation promoted HUVECs cell survival and proliferation. Compared to control, no significant differences were observed in autophagic status of encapsulated cells ( p > 0.05). The level of Tie‐1, Tie‐2, VEGFR‐1, and VEGFR‐2 were increased, but did not reach to significant levels. Encapsulation decreased MMP‐2, ‐9 activity and increased the VE‐cadherin level in enclosed cells ( p < 0.05). Moreover, an enhanced in vivo angiogenic response of encapsulated HUVECs was evident as compared to non‐capsulated cells ( p < 0.05). These observations suggest that alginate‐gelatin encapsulation can induce angiogenic response in in vivo and in vitro conditions.