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Enhanced d ‐lactic acid production by recombinant Saccharomyces cerevisiae following optimization of the global metabolic pathway
Author(s) -
Yamada Ryosuke,
Wakita Kazuki,
Mitsui Ryosuke,
Ogino Hiroyasu
Publication year - 2017
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.26330
Subject(s) - metabolic engineering , lactic acid , fermentation , leuconostoc mesenteroides , saccharomyces cerevisiae , yeast , biochemistry , chemistry , metabolic pathway , food science , yield (engineering) , glycolysis , bacteria , biology , metabolism , enzyme , materials science , metallurgy , genetics
Utilization of renewable feedstocks for the production of bio‐based chemicals such as d ‐lactic acid by engineering metabolic pathways in the yeast Saccharomyces cerevisiae has recently become an attractive option. In this study, to realize efficient d ‐lactic acid production by S. cerevisiae , the expression of 12 glycolysis‐related genes and the Leuconostoc mesenteroides d ‐LDH gene was optimized using a previously developed global metabolic engineering strategy, and repeated batch fermentation was carried out using the resultant strain YPH499/dPdA3‐34/DLDH/1‐18. Stable d ‐lactic acid production through 10 repeated batch fermentations was achieved using YPH499/dPdA3‐34/DLDH/1‐18. The average d ‐lactic acid production, productivity, and yield with 10 repeated batch fermentations were 60.3 g/L, 2.80 g/L/h, and 0.646, respectively. The present study is the first report of the application of a global metabolic engineering strategy for bio‐based chemical production, and it shows the potential for efficient production of such chemicals by global metabolic engineering of the yeast S. cerevisiae . Biotechnol. Bioeng. 2017;114: 2075–2084. © 2017 Wiley Periodicals, Inc.