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Effects of plant density on recombinant hemagglutinin yields in an Agrobacterium ‐mediated transient gene expression system using Nicotiana benthamiana plants
Author(s) -
Fujiuchi Naomichi,
Matsuda Ryo,
Matoba Nobuyuki,
Fujiwara Kazuhiro
Publication year - 2017
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.26303
Subject(s) - nicotiana benthamiana , agrobacterium , recombinant dna , biology , yield (engineering) , botany , hemagglutinin (influenza) , biomass (ecology) , horticulture , agronomy , gene , transgene , biochemistry , physics , thermodynamics
Agrobacterium‐mediated transient expression systems enable plants to rapidly produce a wide range of recombinant proteins. To achieve economically feasible upstream production and downstream processing, it is beneficial to obtain high levels of two yield‐related quantities of upstream production: recombinant protein content per fresh mass of harvested biomass (g gFM −1 ) and recombinant protein productivity per unit area‐time (g m −2 /month). Here, we report that the density of Nicotiana benthamiana plants during upstream production had significant impacts on the yield‐related quantities of recombinant hemagglutinin (HA). The two quantities were smaller at a high plant density of 400 plants m −2 than at a low plant density of 100 plants m −2 . The smaller quantities at the high plant density were attributed to: (i) a lower HA content in young leaves, which usually have high HA accumulation potentials; (ii) a lower biomass allocation to the young leaves; and (iii) a high area‐time requirement for plants. Thus, plant density is a key factor for improving upstream production in Agrobacterium ‐mediated transient expression systems. Biotechnol. Bioeng. 2017;114: 1762–1770. © 2017 Wiley Periodicals, Inc.