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Producing desulfurized biogas through removal of sulfate in the first‐stage of a two‐stage anaerobic digestion
Author(s) -
Yun YeoMyeong,
Sung Shihwu,
Shin HangSik,
Han JongIn,
Kim HyunWoo,
Kim DongHoon
Publication year - 2017
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.26233
Subject(s) - acidogenesis , biogas , sulfate , chemistry , anaerobic digestion , hydraulic retention time , desulfovibrio , pulp and paper industry , bioreactor , anaerobic exercise , sulfate reducing bacteria , waste management , sewage treatment , environmental engineering , methane , environmental science , organic chemistry , biology , engineering , physiology
In the present work, a two‐stage anaerobic digestion system (TSADS) was newly designed to produce biogas with a greatly reduced H 2 S content. The role of first (sulfidogenic)‐stage digester was not only acidogenesis but also sulfidogenesis (sulfate reduction to H 2 S), which would minimize the input of H 2 S‐producing source in the followed second (methanogenic)‐stage digester. For the coexistence of acidogens and sulfate reducing bacteria (SRB) in the sulfidogenic‐stage digester, it was found that pH played a crucial role. The acidogenic activity was not affected within a pH range of 4.5–6.0, while it was important to maintain a pH at 5.5 to achieve a sulfate removal efficiency over 70%. The highest sulfate removal attained was 78% at a hydraulic retention time (HRT) of 5 h at pH 5.5 ± 0.1. The H 2 S content in the biogas produced in the conventional single‐stage digester (SSAD), used as a control, reached 1,650 ± 25 ppm v . In contrast, the biogas produced in the methanogenic‐stage digester of the developed process had an H 2 S content of 200 ± 15 ppm v . Microbial analysis, done by the next generation sequencing technique, clearly showed the changes in community under different operating conditions. Desulfovibrio bastinii (4.9%) played a key role in sulfate removal in the sulfidogenic‐stage of the TSADS owing to its characteristics of a short doubling time and growth in an acidic environment. Biotechnol. Bioeng. 2017;114: 970–979. © 2016 Wiley Periodicals, Inc.