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Biodegradation of nitrobenzene through a hybrid pathway in Pseudomonas putida
Author(s) -
Jung KwanHye,
Lee JangYoung,
Kim HakSung
Publication year - 1995
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260480610
Subject(s) - biodegradation , nitrobenzene , pseudomonas putida , chemistry , environmental chemistry , organic chemistry , enzyme , catalysis
The biodegradation of nitrobenzene was attempted by using Pseudomonas putida TB 103 which possesses the hybrid pathway combining the tod and the tol pathways. Analysis of the metabolic flux of nitrobenzene through the hybrid pathway indicated that nitrobenzene was initially oxidized to cis ‐1,2‐dihydroxy‐3‐nitrocyclohexa‐3,5‐diene by toluene dioxygenase in the tod pathway and then channeled into the tol pathway, leading to the complete biodegradation of nitrobenzene. A crucial metabolic step redirecting the metabolic flux of nitrobenzene from the tod to the tol pathway was determined from the genetic and biochemical studies on the enzymes involved in the tol pathway. From these results, it was found that toluate‐ cis ‐glycol dehydrogenase could convert cis ‐1,2‐dihydroxy‐3‐nitrocyclohexa‐3,5‐diene to catechol in the presence of NAD + with liberation of nitrite and the reduced form of NAD + (NADH) into the medium. © 1995 John Wiley & Sons, Inc.