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Development of surface swabbing procedures for a cleaning validation program in a biopharmaceutical manufacturing facility
Author(s) -
Lombardo Silvia,
Inampudi Prasad,
Scotton Anthony,
Ruezinsky Gary,
Rupp Randall,
Nigam Somesh
Publication year - 1995
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260480514
Subject(s) - biopharmaceutical , manufacturing engineering , engineering , environmental science , biochemical engineering , process engineering , microbiology and biotechnology , biology
Validation of direct surface swabbing procedure in conjuction with total organic carbon (TOC) analysis is described for a biopharmaceutical product manufacturing operation. The swabbing technique was found to be very effective in reliably detecting very low levels of residuals for diverse process streams (limit of detection of approximately 0.5µg/cm 2 ). However, contaminant recovery was significantly dependent on both the type of contaminant and the processing surface. This study serves as a guide for designing effective cleaning validation protocols based on direct surface swabbing techniques. © 1995 John Wiley & Sons, Inc.