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Protected amino acids as novel low‐molecular‐weight displacers in cation‐exchange displacement chromatography
Author(s) -
Kundu Amitava,
Vunnum Suresh,
Jayaraman Guhan,
Cramer Steven M.
Publication year - 1995
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260480507
Subject(s) - displacement chromatography , chemistry , ion chromatography , ion exchange , chromatography , lysine , amino acid , polyelectrolyte , polymer , ion , biochemistry , organic chemistry , high performance liquid chromatography , chromatography column
Although the ability to carry out simultaneous concentration and purification in a single displacement step has significant advantages for downstream processing of pharmaceuticals, a major impediment to the implementation of displacement chromatography has been the lack of suitable displacer compounds. An important recent advance in the state of the art of displacement chromatography has been the discovery that low‐molecular‐weight dendritic polymers can be successfully employed as displacers for protein purification in ion‐exchange systems. In this article, protected amino acid esters (based on arginine and lysine) are shown to be useful displacers for protein purification in cation‐exchange systems. A dynamic affinity plot is employed to evaluate the affinity of these low‐molecular‐weight compounds under dis‐placement conditions. In contrast to large polyelectroyte displacers, the efficacy of these low‐molecular‐weight displacers was shown to be dependent on both the initial carrier salt concentration and the displacer concentration. In addition to the funcamental interest generated by low‐molecular‐weight displacers, it is likely that these displacers will have significant operatioal advantages as compared with large polyelectrolyte displacers. © 1995 John Wiley & Sons, Inc.