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Fed‐batch culture of Bacillus thuringiensis for thuringiensin production in a tower type bioreactor
Author(s) -
Jong JianZhong,
Hsiun DingYu,
Wu WenTeng,
Tzeng YewMin
Publication year - 1995
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260480307
Subject(s) - airlift , bacillus thuringiensis , bioreactor , continuous stirred tank reactor , chemistry , substrate (aquarium) , pulp and paper industry , food science , chromatography , microbiology and biotechnology , biology , botany , bacteria , engineering , ecology , genetics
Fed‐batch culture of Bacillus thuringiensis in a modified airlift reactor has been developed by using adaptive control of glucose concentration in the reactor. The glucose concentration was estimated via a correlation equation between carbon dioxide production rate and glucose consumption rate. The estimated glucose concentration as the output variable was fed back to computer for calculation of substrate addition. The modified reactor was an airlift reactor with a net draft tube. The airlift reactor had high oxygen transfer rate and low shear stress which were important factors for production of thuringiensin. Fed‐batch culture of Bacillus thuringiensis in the modified airlift reactor provided significant improvement of thuringiensin production. © 1995 John Wiley & Sons, Inc.