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Protein‐free human–human hybridoma cultures in an intercalated‐spiral alternate‐dead‐ended hollow fiber bioreactor
Author(s) -
Brotherton John D.,
Chau Pao C.
Publication year - 1995
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260470312
Subject(s) - bioreactor , spiral (railway) , fiber , chromatography , materials science , chemistry , chemical engineering , composite material , engineering , mechanical engineering , organic chemistry
Batch cell cultures of a human‐human hybridoma line in a convective flow dominant intercalated‐spiral altetnate‐dead‐ended hollow fiber are compared with those using conventional axial‐flow hollow fiber bioreactors and a stirred‐tank bioreactor. Relatively short‐term fed‐batch and perfusion cell cultures were also employed for the intercalated‐spiral bioreactor. When operating conditions of a batch intercalated‐spiral bioreactor were properly chosen, the cell growth and substrate consumption paralleled that of a batch stirred‐tank culture. The results verified the premise of the intercalated‐spiral hollow fiber bioreactor that nutrient transport limitations can be eliminated when the convective flux through the extracapillary space is sufficiently high.© John Wiley & Sons, Inc.