Premium
In‐situ removal of ammonium and lactate through electrical means for hybridoma cultures
Author(s) -
Chang YuHsiang David,
Grodzinsky Alan J.,
Wang Daniel I. C.
Publication year - 1995
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260470305
Subject(s) - electrokinetic phenomena , glutamine , ammonium , electric field , electrophoresis , chemistry , chromatography , cell growth , ammonia , suspension (topology) , cell culture , biochemistry , biology , amino acid , organic chemistry , physics , mathematics , quantum mechanics , homotopy , pure mathematics , genetics
Ammonium and lactate are two known toxic products detrimental to mammalian cell growth and productivity. An electrokinetic technique, utilizing an electrophoretic mechanism, was developed to remove these cellular wastes in‐situ from suspension hybridoma (ATCC CRL‐1606) cultures to enhance cell growth and productivity. This technique applies continuously a dc electric field to selectively remove the electrically charged wastes. The experiments were shown to be successful in the removal of externally added 10 rn M ammonium and 45 m M lactate while maintaining the chemostatic condition of culture medium in a cell‐free condition under an electric current density of 50 A/m 2 . Toxic levels of ammonium were added, ranging from 7.5 to 12.5 m M , at the start of the hybridoma culture, and the applied dc electric fields were able to completely remove these added materials. This in turn released the inhibition and restored the cell growth. Finally, this electrokinetic technique was applied to the batch and glutamine fed‐batch hybridoma cultures. At an applied electric current density of 50 A/m 2 , this was able to completely remove cell‐produced ammonium and increased the cell growth and antibody titer by 30% to 50%, respectively, compared to the control experiment in the absence of the electric field. Lastly, the applied electric current density of 50 A/m 2 did not affect cellular functionalities such as glucose and glutamine consumption and antibody productivity.© 1995 John Wiley & Sons, Inc