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Protein recovery from cell debris using rotary and tangential crossflow microfiltration
Author(s) -
Parnham Charles S.,
Davis Robert H.
Publication year - 1995
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260470206
Subject(s) - microfiltration , fouling , filter cake , membrane , filter (signal processing) , chromatography , cross flow filtration , chemistry , flux (metallurgy) , membrane fouling , permeation , filtration (mathematics) , volumetric flow rate , materials science , analytical chemistry (journal) , mechanics , physics , engineering , mathematics , biochemistry , statistics , organic chemistry , electrical engineering
Abstract Protein recovery from a bacterial lysate was accomplished using microfiltration membranes in a flat crossflow filter and in a cylindrical rotary filter. Severe membrane fouling yielded relatively low long‐term permeate flux values of 10 −4 –10 −3 cm/s (where I cm/s = 3.6 × 10 4 L/m 2 – h). The permeate flux was found to be nearly independent of transmembrane pressure and to increase with increasing shear rate and decreasing solids concentration. The flux increased with shear to approximately the one‐third power or greater for the flat filter and the one‐half power or greater for the rotary filter; the stronger dependence for the rotary filter is thought to result from Taylor vortices enhancing the back transport of debris carried to the membrane surface by the permeate flow. The average protein transmission or sieving coefficient was measured at approximately 0.6, but considerable scatter in the transmission data was observed. The largest sieving coefficients were obtained for dilute suspensions at high shear rate. The rotary filter provided higher fluxes than did the flat filter for dilute suspensions, but not for concentrated suspensions. © 1995 John Wiley & Sons, Inc.