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In situ microscopy for on‐line characterization of cell‐populations in bioreactors, including cell‐concentration measurements by depth from focus
Author(s) -
Suhr H.,
Wehnert G.,
Schneider K.,
Bittner C.,
Scholz T.,
Geissler P.,
Jähne B.,
Scheper T.
Publication year - 1995
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260470113
Subject(s) - hemocytometer , in situ , bioreactor , microscopy , microscope , fluorescence microscope , characterization (materials science) , fermentation , yeast , materials science , cell counting , cell culture , chromatography , biological system , biomedical engineering , chemistry , cell , biology , optics , nanotechnology , fluorescence , biochemistry , medicine , physics , organic chemistry , genetics , cell cycle
A new technique is presented which allows the use of a front‐end sensor head for in situ and on‐line characterization of cell concentration and cell size during fermentation. An epifluorescence microscope is mounted in a port of a bioreactor viewing directly into the agitated broth. Still images from cells are generated using pulsed illumination. They are directly visualized on a monitor and used for automatic image analysis. The cell concentration and morphological information are determined by counting and evaluating the cell images with respect to their depth from focus characteristic. An in situ microscope was successfully tested during yeast fermentations and yielded results which correlated well with results from a hemocytometer. © 1995 John Wiley & Sons, Inc.