Premium
Growth kinetics of glucose‐limited petunia hybrida cells in chemostat cultures: Determination of experimental values for growth and maintenance parameters
Author(s) -
de Gucht Luuk P. E.,
van der Plas Linus H. W.
Publication year - 1995
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260470106
Subject(s) - chemostat , adenosine triphosphate , biochemistry , extracellular , mole , biomass (ecology) , kinetics , yield (engineering) , chemistry , growth rate , biology , food science , ecology , genetics , physics , mathematics , quantum mechanics , bacteria , metallurgy , materials science , geometry
With glucose‐limited continuous cultures of Petunia hybrida six steady states were obtained at specific growth rates varying from 0.0035 to 0.012 h −1 (corresponding with culture residence times varying from 285 to 85 h). The macromolecular and the elemental biomass composition which were determined in four steady states showed no major differences over the range of growth rates examined. During all six steady states specific subtrate and oxygen consumption as well as biomass and extracellular product formation rates were monitored. Moreover the specific activities of the mitochondrial cytochrome and alternative pathway were determined and used to estimate specific adenosine triphosphate (ATP) production rates. Data thus obtained were used in the determination of maintenance and true growth yield parameters. For the maintenance on glucose and ATP values of 0.0070 C‐mol/C‐mol/h and 0.034 mol/C‐mol/h were obtained, respectively. True yields of biomass on glucose and ATP were 0.50 C‐mol/C‐mol and 0.28 C‐mol/mol, respectively. © 1995 John Wiley & Sons, Inc.