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Physico‐chemical properties of the encapsulation matrix and germination of carrot somatic embryos
Author(s) -
Timbert Raphaëlle,
Barbotin JeanNoël,
Kersulec Alain,
Bazinet Christophe,
Thomas Daniel
Publication year - 1995
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260460610
Subject(s) - gellan gum , somatic embryogenesis , dehydration , somatic cell , germination , chemistry , polysaccharide , relative humidity , embryo , matrix (chemical analysis) , botany , food science , biology , chromatography , biochemistry , tissue culture , in vitro , microbiology and biotechnology , physics , gene , thermodynamics
Abstract Carrot somatic embryos were encapsulated in alginate gel beads. To improve the quality of a “synthetic seed” coating, the rheology and dehydration properties of different matrices were tested. By increasing alginate and CaCl 2 concentrations, additional mineral elements were shown to increase resistance to rupture, and to depress the germination of somatic embryos. A polysaccharide addition was found to slow the alginate matrix dehydration; alginate‐gellan gum and alginate‐kaolin matrices could preserve the viability of somatic embryos at low relative humidities (30% to 35% germinations at 50% relative humidity) to a greater extent than other matrices. © 1995 John Wiley & Sons, Inc.