Physico‐chemical properties of the encapsulation matrix and germination of carrot somatic embryos | Zendy

Timbert Raphaëlle | Zendy; Barbotin JeanNoël | Zendy; Kersulec Alain | Zendy; Bazinet Christophe | Zendy; Thomas Daniel | Zendy

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Physico‐chemical properties of the encapsulation matrix and germination of carrot somatic embryos

Author(s) -

Timbert Raphaëlle,

Barbotin JeanNoël,

Kersulec Alain,

Bazinet Christophe,

Thomas Daniel

Publication year - 1995

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.260460610

Subject(s) - gellan gum , somatic embryogenesis , dehydration , somatic cell , germination , chemistry , polysaccharide , relative humidity , embryo , matrix (chemical analysis) , botany , food science , biology , chromatography , biochemistry , tissue culture , in vitro , microbiology and biotechnology , physics , gene , thermodynamics

Carrot somatic embryos were encapsulated in alginate gel beads. To improve the quality of a “synthetic seed” coating, the rheology and dehydration properties of different matrices were tested. By increasing alginate and CaCl 2 concentrations, additional mineral elements were shown to increase resistance to rupture, and to depress the germination of somatic embryos. A polysaccharide addition was found to slow the alginate matrix dehydration; alginate‐gellan gum and alginate‐kaolin matrices could preserve the viability of somatic embryos at low relative humidities (30% to 35% germinations at 50% relative humidity) to a greater extent than other matrices. © 1995 John Wiley & Sons, Inc.

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