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Reversed micellar extraction of trypsin: Effect of solvent on the protein transfer and activity recovery
Author(s) -
Chang Qinglong,
Chen Jiayong
Publication year - 1995
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260460210
Subject(s) - chemistry , extraction (chemistry) , trypsin , chromatography , micelle , solvent , pulmonary surfactant , heptane , octane , cyclohexane , hexane , organic chemistry , biochemistry , aqueous solution , enzyme
By using trypsin as the model protein and AOT as the model surfactant, the effect of a variety of solvents on protein transfer and activity recovery during the liquid–liquid reversed micellar extraction was investigated. It was found that several solvents, including isooctane, octane, heptane, and kerosene, had a similar effect on the recovery of trypsin activity after a full cycle of forward and backward extraction, and could all be used as the solvents for AOT‐reversed micelles in trypsin extraction. Two other solvents (hexane and cyclohexane), however, were not so efficient. © 1995 John Wiley & Sons, Inc.