Premium
Photolithotrophic cultivation of Laminaria saccharina gametophyte cells in a stirred‐tank bioreactor
Author(s) -
Qi Hans,
Rorrer Gregory L.
Publication year - 1995
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260450310
Subject(s) - saccharina , bioreactor , laminaria , gametophyte , botany , aeration , zoology , saturation (graph theory) , biomass (ecology) , biology , inoculation , fermentation , chemistry , algae , horticulture , food science , ecology , pollen , mathematics , combinatorics
Filamentous cell cultures derived from female gametophytes of the temperate brown macroalga Laminaria saccharina were photolithotrophically cultivated in artificial seawater medium within an illuminated 1.3‐L stirred‐tank bioreactor at 13°C using CO 2 in air as the carbon source. A Monod model adequately described light‐saturated growth. The apparent half‐saturation constant ( K o ) was 23 μE/m 2 ‐s, and maximum specific growth rate was 0.15 day −1 . At a constant inoculation cell density of 50 mg DCW/L, biomass productivity after 26 days of cultivation increased from 630 mg DCW/L at 18 μE/m 2 ‐s to 890 mg DCW/L at 228 μE/m 2 ‐s. At 98 μE/m 2 ‐s, 1.1 vvm aeration rate, and 250 rpm impeller speed, the CO 2 transfer rates (CO 2 TRs) and CO 2 consumption rates ( r co 2) were determined over the cultivation period. At peak CO 2 demand, the maximum CO 2 TR was 0.19 mmol CO 2 /L‐h, but r co 2was only 0.15 mmol CO 2 /L‐h, implying that the culture was not CO 2 transport limited. This is the first reported bioreactor cultivation study of cell cultures derived from a macrophytic marine alga. © 1995 John Wiley & Sons, Inc.