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Serum‐free cell culture on insulin‐immobilized porous collagen beads
Author(s) -
Ito Yoshihiro,
Zheng Ji,
Imanishi Yukio
Publication year - 1995
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260450208
Subject(s) - carbodiimide , chemistry , insulin , albumin , adhesion , bead , bovine serum albumin , chromatography , biochemistry , biology , endocrinology , materials science , organic chemistry , composite material
Insulin or albumin was immobilized on collagen beads using water‐soluble carbodiimide. Adhesion of STO mouse fibroblast cells onto the beads decreased with increasing the amount of immobilized proteins. Growth of the cells was remarkably accelerated on the insulinimmobilized collagen beads, which can be used for serum‐free cell culture. The growth acceleration became larger with increasing the amount of immobilized insulin, while it became smaller with increasing the amount of immobilized albumin. In addition, the immobilized insulin more strongly accelerated the cell growth than free insulin plus collagen beads. © 1995 John Wiley & Sons, Inc.