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Synthesis of protein‐containing polymers in organic solvents
Author(s) -
Yang Zhen,
Williams Darrel,
Russell Alan J.
Publication year - 1995
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260450103
Subject(s) - subtilisin , polymer , polyethylene glycol , chemistry , peg ratio , polymerization , polymer chemistry , methacrylate , aqueous solution , organic chemistry , enzyme , finance , economics
Subtilisin has been modified with polyethylene glycol (PEG) monomethacrylate (MW 8000) by reductive alkylation, and incorporated into polymethyl methacrylate durring free‐radical initiated polymerization. The activity and stability of the PEG‐modified enzymes have been determined in aqueous buffer and organic solvents. The K m and V max values for unmodified, singly and doubly modified subtilisin were compared in these environments, and the half‐lives of both modified enzymes were remarkably high (up to 2 months). The protein‐containing polymer was analyzed for activity and polymer properties, and our results indicate that active subtilisin can be incorporated into polymethyl methacrylate during polymerization in organic solvents while retaining its activity and stability. © 1995 John Wiley & Sons, Inc.