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Viability and protein secretion from human Hepatoma (HepG2) cells encapsulated in 400‐μm polyacrylate microcapsules by submerged nozzle–liquid jet extrusion
Author(s) -
Uludag Hasan,
Horvath Vlad,
Black John P.,
Sefton Michael V.
Publication year - 1994
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260441007
Subject(s) - methacrylate , chemistry , extrusion , polymer , secretion , shearing (physics) , chromatography , membrane , nozzle , polymer chemistry , biophysics , materials science , chemical engineering , biochemistry , monomer , organic chemistry , composite material , physics , biology , engineering , thermodynamics
An interfacial precipitation process to encapsulate mammalian cells in hydroxyethyl methacrylate‐methyl methacrylate (HEMA‐MMA) microcapsules of ∼ 750 in ∼m diameter was previously described. It was not possible to produce smaller capsules due to low shearing force. A new droplet generation scheme was developed by suspending the cell and polymer co‐extrusion nozzle in a uniform co‐axial fluid jet which enabled the production of 300 to 600‐µm diameter capsules. HepG2 hepatoma cells in 400‐µm‐diameter HEMA‐MMA capsules were able to retain their metabolic activity during and after the encapsulation process. The in vitro secretion of plasma proteins α 1 ‐acid glycoprotein, α 1 ‐antitrypsin, and fibrinogen by the encapsulated cells was retained. The encapsulated cells secreted less fibrinogen (340 kD) relative to α 1 ‐acid glycoprotein (42kD), indicating the sieving effect (but not absolute cut‐off) of the HEMA‐MMA membrane. © 1994 John Wiley & Sons, Inc.