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Physiological responses of hybridoma cells in a protein‐free medium to soluble and immobilized antigen
Author(s) -
Dandulakis Gregory,
Herr John C.,
Kirwan Donald J.
Publication year - 1994
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260440917
Subject(s) - antigen , monoclonal antibody , antibody , sepharose , chemistry , microbiology and biotechnology , cell culture , biology , biochemistry , chromatography , immunology , enzyme , genetics
This study examined the effect of antigen in a protein free medium on cell growth and monoclonal antibody production by a hybridoma line. Antigen immobilized on a Sepharose gel matrix via a bovine γ‐globulin carrier protein was used to stimulate the cell cultures in T‐flasks. In comparison to antigen‐free culture, total antibody production during was increased up to 40%, while slower cell growth rates were observed. The specific antibody production during the stationary culture phase was 40% to 80% higher in the presence of immobilized antigen. The surface density of antigen on the Sepharose beads had a strong influence on the physiological response of the hybridomas. © 1994 John Wiley & Sons, Inc.