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The influence of dissolved oxygen tension on the synthesis of the antibiotic difficidin by bacillus subtilis
Author(s) -
Suphantharika M.,
Ison A. P.,
Lilly M. D.,
Buckland B. C.
Publication year - 1994
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260440818
Subject(s) - bacillus subtilis , saturation (graph theory) , oxygen , chemistry , oxygen tension , bacteria , organic chemistry , biology , genetics , mathematics , combinatorics
The antibiotic, difficidin, and its hydroxylated derivative oxydifficidin, were synthesized by cultures of Bacillus subtilis grown on a complex medium. Maximum titers of about 200 and 130 mg/L, respectively, were obtained. In fermentations where the dissolved oxygen tension (DOT) was controlled, the maximum specific growth rate was only reduced below 5% air saturation. DOT had little effect on the volumetric rateof synthesis of oxydifficidin but greatly influenced the rate for difficidin, which was reduced at DOT values below 40% air saturation. © 1994 John Wiley & Sons, Inc.