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Hyperosmotic hbridoma cell cultures: Increased monoclonal antibody production with addition of glycine betaine
Author(s) -
Øyaas Karin,
Ellingsen Trond E.,
Dyrset Nils,
Levine David W.
Publication year - 1994
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260440816
Subject(s) - betaine , osmotic concentration , glycine , sucrose , titer , proline , biochemistry , cell culture , osmolyte , osmotic pressure , biology , antibody , chemically defined medium , sarcosine , chemistry , microbiology and biotechnology , amino acid , in vitro , immunology , genetics
When mouse hybridoma cells were grown in culture media which were made hyperosmotic through the addition of NaCl or sucrose, the specific rate of antibody production increased with medium osmolality, reaching approx. 1.9 times the level obtained at physiological osmolality. However, due to a simultaneous reduction of the maximal cell density in the hyperosmotic media, the effect of the increased production rate did not give significant increases in the maximum antibody titer obtained in the cultures. When the osmoprotective compound, glycine betaine, was included in the NaCl‐ or sucrose‐stressed cultures, the specific antibody production rate wasincreased up to 2.6‐fold and maximum antibody titer up to twofold over that obtained in the control culture (physiological osmolality). A similar pattern of response was observed when other osmoprotective compounds (sarcosine, proline, glycine) were added to NaCl‐stressed hybridoma cell cultures. For the present experiments, the results suggest that medium osmolality, rather than growth rate, will determine the specific antibody production rate by hybridoma cell line 6H11 growing in hyperosmotic culture media. © 1994 John Wiley & Sons, Inc.