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Formation of carotenoids by rhodotorula glutinis in whey ultrafiltrate
Author(s) -
Frengova Ginka,
Simova Emilina,
Pavlova Konstantza,
Beshkova Dora,
Grigorova Drinka
Publication year - 1994
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260440804
Subject(s) - rhodotorula , carotenoid , chemistry , food science , chromatography , biochemistry , yeast
The growth and carotenoid biosynthesis of the yeast Rhodotorula glutinis was studied by cocultivation with Lactobacillus helveticus in cheese ultrafiltrate containing 3.9% and 7.1% lactose. By growing this mixed culture in a 15‐L fermentor MBR AG (Switzerland) at an air flow rate of 0.5 L/L min and agitation at 220 rpm for 6 days, a total yield of carotenoids of 268 μg/g dry cells wasobtained. Carotenoids were formed almost parallel with the cell growth, anda maximum production was reached at an early stationary phase. A high‐performance liquid chromatographic system (HPLC) permitting simultaneous determination of major carotenoid pigments was used. The three main pigments (torularhodin, β‐carotene, and torulene) were formed in Rhodotorula glutinis , and reached a maximum concentration as follows: 182.0, 43.9, 23.0 μg,g dry cells. © 1994 John Wiley & Sons, Inc.