Premium
Improving protein extraction yield in reversed micellar systems through surface charge engineering
Author(s) -
Pires M. J.,
Martel P.,
Baptista A.,
Petersen S. B.,
Willson R. C.,
Cabral J. M. S.
Publication year - 1994
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260440702
Subject(s) - yield (engineering) , extraction (chemistry) , chemistry , surface charge , charge (physics) , chromatography , biological system , materials science , biochemical engineering , engineering , biology , physics , composite material , quantum mechanics
The mechanism of extraction of rat cytochrome b 5 from water into a sodium dioctylsulfosuccinate (AOT) micellar organic phase was studied using protein engineering of surface charged residues. The extraction behavior of native cytochrome b 5 and modified proteins with substitutions of the type glutamic acid → lysine at positions 44 (E44K), 56 (E56K), and 92 (E92K), was studied as a function of pH. The results indicate that an important mechanism of extraction is an electrostatic interaction of this protein with the negatively charged surfactant. We demonstrate that it is possible to improve extraction by engineering the protein surface charge, increasing the driving force responsible for the protein transfer to the micellar phase. © 1994 John Wiley & Sons, Inc.