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The release of virus‐like particles from recombinant Saccharomyces cerevisiae : Effect of freezing and thawing on homogenization and bead milling
Author(s) -
Milburn P. T.,
Dunnill P.
Publication year - 1994
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260440610
Subject(s) - saccharomyces cerevisiae , homogenizer , homogenization (climate) , chemistry , recombinant dna , virus like particle , freeze drying , biophysics , biology , biochemistry , yeast , chromatography , gene , biodiversity , ecology
Recombinant cells of Saccharomyces cerevisiae , expressing virus‐like particles (Ty‐VLPs), can be readily disrupted in a high pressure homogenizer and show identical disruption kinetics to the untransformed host strain. When the cells are freeze/thawed before disruption, they become about four times more resistant to homogenization. This effect increases with the number of freeze/thaw cycles, but is independent of the time the cells remain frozen. The freeze/thaw effect is observed with cells harvested during both the logarithmic and stationary phase of growth, and occurs with the untransformed host strain as well as the transformed one. Freeze/thawed cells are twice as resistant to disruption in the bead mill as fresh cells. © 1994 John Wiley & Sons, Inc.