A quantitative analysis of shear effects on cell suspension and cell culture of  perilla frutescens in bioreactors | Zendy

Zhong JianJiang | Zendy; Fujiyama Kazuhito | Zendy; Seki Tatsuji | Zendy; Yoshida Toshiomi | Zendy

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A quantitative analysis of shear effects on cell suspension and cell culture of perilla frutescens in bioreactors

Author(s) -

Zhong JianJiang,

Fujiyama Kazuhito,

Seki Tatsuji,

Yoshida Toshiomi

Publication year - 1994

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.260440512

Subject(s) - perilla frutescens , suspension culture , bioreactor , perilla , suspension (topology) , cell culture , chemistry , chromatography , botany , biology , food science , mathematics , organic chemistry , genetics , raw material , homotopy , pure mathematics

The short‐time effects of shear on suspended cells of Perilla frutescens were quantitatively analyzed by exposing the cells to a well‐defined flow field in a rotating drum reactor. It was found that both shear rate and shearing time significantly affected cell viability. The quantitative effects of shear on cell growth and the production of anthocyanin, a secondary metabolite, by the cell cultures were further investigated in a series of batch cultivations using a 5‐L plant cell bioreactor with a marine impeller. The results indicated that there was an optimum range of shear rate; i.e., an average shear rate of 20 to 30 s −1 or an impeller tip speed of 5 to 8 dm/s, which maximized all the values of the following parameters: the specific growth rate, the maximum cell concentration, the (specific) production and productivity of anthocyanin, and the cell and anthocyanin yields. © 1994 John Wiley & Sons, Inc.

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