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A quantitative analysis of shear effects on cell suspension and cell culture of perilla frutescens in bioreactors
Author(s) -
Zhong JianJiang,
Fujiyama Kazuhito,
Seki Tatsuji,
Yoshida Toshiomi
Publication year - 1994
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260440512
Subject(s) - perilla frutescens , suspension culture , bioreactor , perilla , suspension (topology) , cell culture , chemistry , chromatography , botany , biology , food science , mathematics , organic chemistry , genetics , raw material , homotopy , pure mathematics
Abstract The short‐time effects of shear on suspended cells of Perilla frutescens were quantitatively analyzed by exposing the cells to a well‐defined flow field in a rotating drum reactor. It was found that both shear rate and shearing time significantly affected cell viability. The quantitative effects of shear on cell growth and the production of anthocyanin, a secondary metabolite, by the cell cultures were further investigated in a series of batch cultivations using a 5‐L plant cell bioreactor with a marine impeller. The results indicated that there was an optimum range of shear rate; i.e., an average shear rate of 20 to 30 s −1 or an impeller tip speed of 5 to 8 dm/s, which maximized all the values of the following parameters: the specific growth rate, the maximum cell concentration, the (specific) production and productivity of anthocyanin, and the cell and anthocyanin yields. © 1994 John Wiley & Sons, Inc.