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Metabolic monitoring by using the rate of change of NAD(P)H fluorescene
Author(s) -
Kwong Simon C. W.,
Rao Govind
Publication year - 1994
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260440408
Subject(s) - nad+ kinase , corynebacterium glutamicum , derivative (finance) , biochemistry , chemistry , fermentation , intracellular , biology , enzyme , financial economics , economics , gene
The amino acid fermentation by Corynebacterium glutamicum was monitored with an new technique that uses the first derivative of the NAD(P)H fluorescene signal. The rate of change of NAD(P)H pools is indicative of intracellular redox balance variations that correspond to metabolic changes. The profile of this signal showed several characteristics that coincided with major metabolic events during fermentation. We show here that the derivative fluorescence signal can accurately estimate points of threonine depletion, viable cell count, and the end of amino acid formation. Furthermore, on‐line optimization strategies can be developed by using the derivative fluorescene signal. © 1994 John Wiley & Sons, Inc.