Premium
Partitioning of pristinamycins in aqueous two‐phase systems: A first step toward the development of antibiotic production by extractive fermentation
Author(s) -
Paquet V.,
Myint M.,
Roque C.,
Soucaille P.
Publication year - 1994
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260440407
Subject(s) - partition coefficient , peg ratio , polyethylene glycol , fermentation , chemistry , aqueous solution , molar mass , dextran , aqueous two phase system , chromatography , partition (number theory) , organic chemistry , polymer , mathematics , finance , combinatorics , economics
The partitioning of pristinamycins was studied in dextran and polyethylene glycol (PEG) aqueous two‐phases systems. Pristinamycins partitioned preferentially into the PEG‐rich top phase. The partition coefficient was independent of molar mass of PEG and dextran and of antibiotic concentration, but, increased exponentially with the tieline length of the system. Partition of pristinamycins was greatly improved when fatty acids esters of PEG were mixed with PEG. In such mixtures, the partition of coefficient increased up to a value of 24, dependent on the carbon chain length of fatty acids and the modified PEG concentrations. Moreover, in such system, the two groups of pristinamycins, I and II, were extracted in accordance with their hydrophobicity. Recovery of pristinanamycins produced by Streptomyces pritinaespiralis in a fermentation broth was achieved with a dextran/PEG system. Cells were confined into the bottom phase and pristinamycins partitioned in the top phase. However, due to binding of the pristinamycins to the cells, the partition coefficient was slightly lower than of pure antibiotics solutions. © 1994 John Wiley & Sons, Inc.