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Modification of islet of langerhans surfaces with immunoprotective poly(ethylene glycol) coatings via interfacial photopolymerization
Author(s) -
Sawhney Amarpreet S.,
Pathak Chandrashekhar P.,
Hubbell Jeffrey A.
Publication year - 1994
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260440317
Subject(s) - photopolymer , ethylene glycol , islet , polymer chemistry , copolymer , materials science , chemistry , chemical engineering , polymer science , polymerization , polymer , organic chemistry , biology , insulin , endocrinology , engineering
Abstract Poly(ethylene glycol) (PEG) has been used previously to alter immune interactions and systemic clearance of therapeutic proteins. We present herein chemical approaches for the conceptually similar treatment of therapeutic cells and tissues whereby immune and cell adhesive interactions may be reduced or interrupted, in the context of the transplantation of xenogeneic islets of Langerhans for the treatment of insulin‐dependent diabetes mellitus. Visible‐light‐initiated interfacial photopolymerization of multifunctional PEG‐based macromers was performed directly upon the surface of rat islets of Langerhans to produce conformal barrier hydrogel coatings with thickness of order 10 μ;m. The islets continued to be normal in ultrastructure and function as reflected by response to a glucose challenge in vitro. © 1994 John Wiley & Sons, Inc.