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Monitoring of ethanol during fermentation of a lignocellulose hydrolysate by on‐line microdialysis sampling, column liquid chromatography, and an alcohol biosensor
Author(s) -
Buttler Torbjörn,
Gorton Lo,
Jarskog Helena,
MarkoVarga György,
HahnHäGerdal Bärbel,
Meinander Nina,
Olsson Lisbeth
Publication year - 1994
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260440309
Subject(s) - chromatography , microdialysis , chemistry , hydrolysate , amperometry , biosensor , fermentation , alcohol , ethanol , ethanol fermentation , biochemistry , hydrolysis , electrode , extracellular , electrochemistry
Abstract During a 70‐h fermentation of a lignocellulose hydrolysate, the ethanol produced was monitored on‐line using a microdialysis probe as an in situ sampling device. The dialysate components were then separated in a column liquid chromatographic system and the ethanol was selectively detected by an amperometric alcohol biosensor. The result was compared with two off‐line analysis methods: one chromatographic method with refractive index (RI) detection and one enzymatic method based on spectrophotometric detection. The two methods base on enzymes were shown to give lower values than the chromatographic method based on RI detection, which is discussed n terms of selectivity. The investigated on‐line setup was found to be a flexible system for monitoring of fermentations, allowing a sampling frequency of at least 12 h −1 and with a delay between sampling and detection of less than 5 min. © 1994 John Wiley & Sons, Inc.