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Microfiltration of streptococcal fermentation broths: Study of the factors affecting the concentration effect
Author(s) -
HernandezPinzon Immaculada,
Bautista Juan
Publication year - 1994
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260440303
Subject(s) - microfiltration , ultrafiltration (renal) , chemistry , membrane , fermentation , chromatography , permeation , concentration polarization , cross flow filtration , filtration (mathematics) , food science , biochemistry , statistics , mathematics
Streptokinase (SK) recovery from streptococcal fermentation broth by cross‐flow microfiltration has been studied. Recovery of SK in the filtrate, independent of the volumetric concentration factor, is approximately two‐fold lower than the initial SK activity in the fermentation broth; moreover, the SK activity in the retentate increase during the process, reaching a concentration factor of 2.73. These results show that the membrane works more as an ultrafiltration membrane, with rejection of S = 0.6, than as a microfiltration membrane. Under filtration conditions, the membrane permeation rate decreased with time. This decreased could be explained by deposition and interaction of material onto/with the membrane resulting in the concentration of permeable products. Studies of the individual concentration factors for the main streptococcal exocellular proteins, indicate clearly that the concentration of the proteins during the microfiltration process is independent of the size of the proteins, suggesting that other factors, such as charge and hydrophobicity, along with concentration‐polarization, should be taken also into account for the understanding of this phenomenon. © 1994 John Wiley & Sons, Inc.