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Biodegradation process development using a bacterial cytochrome in vivo
Author(s) -
Horowitz Jeffrey B.,
Vilker Vincent L.
Publication year - 1994
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260440214
Subject(s) - pseudomonas putida , chemostat , biodegradation , chemistry , biomass (ecology) , cytochrome , substrate (aquarium) , degradation (telecommunications) , wastewater , bioreactor , oxygen tension , chromatography , bacteria , environmental chemistry , pulp and paper industry , microbiology and biotechnology , biochemistry , enzyme , oxygen , biology , environmental engineering , organic chemistry , environmental science , ecology , telecommunications , computer science , engineering , genetics
Pseudomonas putida PpG786 that contains the inducible enzyme system cytochrome P‐450 cam is considered for use as specialized biomass fore detoxification of hazardous hydrocarbons. The test substrate 1,2‐dibromochloropropane (DBCP) is used to assess the organohalide degradation activity of P. Putida PpG786. Activity was found to be a strong function of intracellular heme content, variables which affect the culturing and processing of the cells, and oxygen tension in the degradation incubation medium. The lifetime for maintaing active biomass in chemostat washout operation, after including substrate was removed and then restarted, was also studied. These results indicate that initial activity of the P. Putida biomass is high enough, and decays slowly enough, so that industrial wastewater treatment at the operating conditions of a sequencing batch reactor (SBR) could remove hazardous compounds. © 1994 John Wiley & Sons, Inc.