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Modeling approach to control of carbohydrate metabolism during citric acid accumulation by Aspergillus niger : II. Sensitivity analysis
Author(s) -
Torres Néstor V.
Publication year - 1994
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260440116
Subject(s) - aspergillus niger , citric acid , hexokinase , biochemistry , metabolism , metabolite , chemistry , flux (metallurgy) , enzyme , carbohydrate metabolism , glycolysis , organic chemistry
Steady state sensitivity analysis of a model of carbohydrate metabolism and anaplerotic synthesis of oxalacetate were, in Aspergillus niger under conditions of citric acid accumulation, carried out. The flux and metabolite concentration control structure of the system obtained shows that the hexokinase/substrate transport step is the main controlling step of the pathway. The quantitative contribution of the other enzyme catalyzed or transport steps are also discussed. These results allow the design of a proper strategy of biotechnological manipulation aimed at improvement of the process. © 1994 John Wiley & Sons, Inc.