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Production of lignin peroxidase by Phanerochaete chrysosporium immobilized on porous poly(styrene‐divinylbenzene) carrier and its application to the degrading of 2‐chlorophenol
Author(s) -
Ruckenstein Eli,
Wang XiaoBai
Publication year - 1994
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260440112
Subject(s) - phanerochaete , chrysosporium , lignin , divinylbenzene , styrene , peroxidase , lignin peroxidase , porosity , chemistry , biodegradation , organic chemistry , chemical engineering , enzyme , copolymer , polymer , engineering
Porous poly(styrene‐divinylbenzene) carriers, for the immobilization of white rot fungus Phanerochaete chrysosporium have been prepared by the concentrated emulsion polymerization method. The concentrated emulsion consists of a mixture of styrene and divinylbenzene containing a suitable surfactant and an initiator as the continuous phase, and water as the dispersed phase. The polymerization of the monomers of the continuous phase generated the polymer carrier with a porcus structure. The white rot fungus Phanerochaete chrysosporium has been immobilized on porous poly(styrene–divinylbenzene) carriers and used for the batch production and the repeated batch production of lignin peroxidase in shake cultures based on a carbon‐limited medium containing veratryl alcohol. The best results were achieved when a spore inoculum was used for immobilization instead of 1‐day‐old mycelial pellets, for both the batch production and the repeated batch production. The porous poly(styrene–divinylbenzene) immobilized Phanerochaete chrysosporium and freely suspended mycelial pellets were used as biocatalysts for the degradation of 2‐chilorophenol in a 2‐L bioreactor. The porous poly(styrene‐divinylbenzene) particle (diameter ≅ 0.2 cm) immobilized spores exhibited a much higher activity in the degradation of 2‐chlorophenol than the freely suspended mycelial pellets. © 1994 John Wiley & Sons, Inc.