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Ethanol production from nonsterilized carob pod extract by free and immobilized Saccharomyces cerevisiae cells using fed‐batch culture
Author(s) -
Roukas T.
Publication year - 1994
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260430302
Subject(s) - ethanol , saccharomyces cerevisiae , fermentation , ethanol fuel , chemistry , bioreactor , sugar , fed batch culture , yeast , food science , biochemistry , chromatography , substrate (aquarium) , biology , organic chemistry , ecology
The production of ethanol from carob pod extract by free and immobilized Saccharomyces cerevisiae cells in batch and fed‐batch culture was investigated. Fed‐batch culture proved to be a better fermentation system for the production of ethanol than batch culture. In fed‐batch culture, both free and immobilized S. cerevisiae cells gave the same maximum concentration (62 g/L) of final ethanol at an initial sugar concentration of 300 g/L and F = 167 mL/h. The maximum ethanol productivity (4.4 g/L h) was obtained with both free and immobilized cells at a substrate concentration of 300 g/L and F = 334 mL/h. In repeated fed‐batch culture, immobilized S. cerevisiae cells gave a higher overall ethanol concentration compared with the free cells. The immobilized S. cerevisiae cells in Ca‐alginate beads retained their ability to produce ethanol for 10 days. © 1994 John Wiley & Sons, Inc.