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A new visualization chamber to study the transient volumetric response of yeast cells submitted to osmotic shifts
Author(s) -
Berner J.L.,
Gervais P.
Publication year - 1994
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260430210
Subject(s) - yeast , tonicity , volume (thermodynamics) , saccharomyces cerevisiae , membrane , chemistry , biophysics , visualization , kinetics , cell , constant (computer programming) , chromatography , thermodynamics , biology , biochemistry , physics , computer science , quantum mechanics , artificial intelligence , programming language
A visualization chamber has been developed to analyze potential correlations between osmotic step increase on yeasts and the resultant cell volume decreases. Image analysis was used to characterize the step increases in the center of the chamber and to measure the changes in the cell volume. Step increases of different intensities have been performed on the yeast Saccharomyces cerevisiae . This device has allowed the kinetics of the volumetric evolution of the cells to be observed. The water exit flow rate from the cell was found to occur in the first 10 s following the hypertonic step change. Comparison of the time constants of the chamber and of the cell volume variations allowed to conclude that the time constant of the water transfer across the membrane was short (about 1 s). © 1994 John Wiley & Sons, Inc.