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Poly(2‐ethyloxazoline) as matrix for highly active electrospun enzymes in organic solvents
Author(s) -
Plothe Ramona,
Sittko Ina,
Lanfer Franziska,
Fortmann Maximilian,
Roth Meike,
Kolbach Vivien,
Tiller Joerg C.
Publication year - 2017
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.26043
Subject(s) - candida antarctica , electrospinning , nanofiber , polymer , spinning , lipase , chemistry , aqueous solution , chemical engineering , polymer chemistry , enzyme , organic chemistry , engineering
ABSTRACT Nanofibers are advantageous carriers for biocatalysts, because they show lower diffusion limitations due to their high surface/volume ratio. Only a few samples are known where enzymes are directly spun into nanofibers, mostly because there are not many suited polymer carriers. In this study, poly(2‐ethyloxazoline) (PEtOx) was explored regarding its usefulness to activate various enzymes in organic solvents by directly electrospinning them from aqueous solutions containing the polymer. It was found that the concentration of PEtOx in the spinning solution and also the swellability of the fibers play a great role in the activity of the enzymes in organic solvents. Using electrospun lipase B from Candida antarctica (CaLB) under optimized conditions revealed a higher carrier activity than the commercial Novozyme 435 with 10 times less immobilized protein. The electrospinning of PEtOx/CaLB fibers onto a stirrer is used to realize a biocatalytic stirrer for organic solvents. Biotechnol. Bioeng. 2017;114: 39–45. © 2016 Wiley Periodicals, Inc.