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Morphological characterization of yeast by image analysis
Author(s) -
Pons M. N.,
Vivier H.,
Rémy J. F.,
Dodds J. A.
Publication year - 1993
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260421112
Subject(s) - budding yeast , staining , yeast , budding , population , morphology (biology) , chemistry , ellipsoid , methylene blue , microscopy , saccharomyces cerevisiae , biology , optics , biochemistry , microbiology and biotechnology , physics , genetics , demography , sociology , photocatalysis , astronomy , catalysis
A semiautomatic image analysis method, with minimal operator intervention, has been developed to characterize the morphology of yeast cells under the assumption that they have an ellipsoidic shape. The cells are observed by optical microscopy and the surface and the minor and major half‐axes of the projection of the ellipsoid on the image plane are determined. Using this method, yeast size distributions and population kinetics (single and budding cells, cell clusters) are determined during alcoholic fermentations. Combination of image analysis with a methylene blue viability test is examined but the staining procedure induces a change in the size of the cells. © 1993 John Wiley & Sons, Inc.