Premium
Membrane formation by interfacial cross‐linking of chitosan for microencapsulation of Lactococcus lactis
Author(s) -
Groboillot A. F.,
Champagne C. P.,
Darling G. D.,
Poncelet D.,
Neufeld R. J.
Publication year - 1993
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260421005
Subject(s) - chitosan , lactococcus lactis , membrane , glutaraldehyde , chemistry , lactic acid , polymerization , chemical engineering , bacteria , polymer chemistry , chromatography , biochemistry , organic chemistry , polymer , biology , engineering , genetics
Lactic acid bacteria were microencapsulated within cross‐linked chitosan membranes formed by emulsification/interfacial polymerization. The technique was modified and optimized to provide biocompatible conditions during encapsulation involving the use of mineral oils as the continuous phase and chitosan as the membrane material. Chitosan cross‐linked with hexamethylene diisocyanate or glutaraldehyde resulted in strong membranes, with a narrow size distribution about a mean diameter of 150 μm. Cell viability and activity was demonstrated by the acidification of milk. Loss of acidification activity during microencapsulation was recovered in subsequent fermentations to levels similar to that of free cell fermentations. © 1993 John Wiley & Sons, Inc.